Prunus serrulata var. spontanea inhibits mast cell activation and mast cell-mediated anaphylaxis.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE\nA promising approach to treat a variety of diseases are considered as complementary and alternative herbal medicines. Prunus serrulata var. spontanea L. (Rosaceae) is used as herbal medicine to treat allergic diseases according to the Donguibogam, a tradition medical book of the Joseon Dynasty in Korea.\n\n\nAIM OF THE STUDY\nWe prepared the aqueous extract of the bark of P. serrulata (AEBPS) and aimed to investigate the effects in mouse anaphylaxis models and various types of mast cells, including RBL-2H3, primary cultured peritoneal and bone marrow-derived mast cells.\n\n\nMATERIALS AND METHODS\nWe used ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) and immunoglobulin (Ig) E-mediated passive cutaneous anaphylaxis (PCA) models, in vivo. The control drug dexamethasone (10\u202fmg/kg) was used to compare the effectiveness of AEBPS (1-100\u202fmg/kg). In vitro, IgE-stimulated mast cells were used to confirm the role of AEBPS (1-100\u202fμg/mL). For statistical analyses, p values less than 0.05 were considered to be significant.\n\n\nRESULTS\nIn ASA model, oral administration of AEBPS suppressed the hypothermia and increased level of serum histamine in a dose-dependent manner. AEBPS attenuated the serum IgE, OVA-specific IgE, and interleukin (IL)-4. Oral administration of AEBPS also blocked mast cell-dependent PCA. AEBPS suppressed degranulation of mast cells by reducing intracellular calcium level in mast cells. AEBPS inhibited tumor necrosis factor-α and IL-4 expression and secretion in a concentration-dependent manner through the reduction of nuclear factor-κB.\n\n\nCONCLUSIONS\nOn the basis of these findings, AEBPS could serve as a potential therapeutic target for the management of mast cell-mediated allergic inflammation and as a regulator of mast cell activation.