Draft genome sequence of broad-spectrum antifungal-producing Bacillus velezensis C4341 isolated from a saline-alkali soil sample in China.

Abstract

OBJECTIVES\nBacterial micro-organisms from the genus Bacillus have a broad spectrum of antimicrobial activity against plant pathogenic bacteria, fungi and viruses. Bacillus velezensis has been extensively used as a biocontrol agent in the agricultural field. In the present study, B. velezensis strain C4341 was isolated from saline-alkali soil in Xinjiang, China, producing a wide range of antifungal compounds of different structures.\n\n\nMETHODS\nThe whole genome of B. velezensis C4341 was sequenced using an Illumina HiSeq™ 2000 sequencing system, was assembled using SOAPdenovo and was optimised with GapCloser.\n\n\nRESULTS\nThe genome size of strain C4341 was calculated at 3 963 750bp with a G+C content of 46.4%. The whole genome was assembled into 4019 open reading frames (ORFs), of which 5.9% and 1.6% were related to antagonistic secondary metabolites and antimicrobial resistance, respectively.\n\n\nCONCLUSION\nThe whole genome sequence of B. velezensis C4341 will be beneficial in comparative analysis of the genus Bacillus and shows future prospects in antifungal application for sustainable agriculture.