Draft genome sequence of a multidrug-resistant blaOXA-69-producing Acinetobacter baumannii L13 isolated from Tarim River sample in China.

Abstract

OBJECTIVES\nAcinetobacter baumannii is the world s most significant opportunistic pathogen causing nosocomial infections, which arouses a widespread health concern due to its ability to acquire multidrug resistance. Here we reported a new variety of A. baumannii L13 isolated from Tarim River in Xinjiang Uygur Autonomous Region of China, with β-lactamase resistance gene OXA-69 in the genome.\n\n\nMETHODS\nGenomic DNA was extracted using an E.Z.N.A. Bacterial DNA Kit according to the protocol recommended by the manufacturer. The genome of A. baumannii L13 was sequenced using an Illumina HiSeqTM 2000 sequencing system, assembled with SOAPdenovo and optimized with GapCloser. The rRNAs were predicted by barrnap and tRNAs were predicted by tRNA-scan-SE. Resistance genes were analyzed using CARD databases.\n\n\nRESULTS\nThe whole genome size of A. baumannii L13 was calculated at 3,969,074 base pairs (bp), which was assembled into 60 contigs and 56 scaffolds (>1,000 bp length), with a G\u2009+\u2009C content of 38.9% and N rate of 0.001%. The number of CDSs was 3,815, with length accounted for approximately 85.61% of the whole genome, 7.6% and 8.2% of which were related to virulence genes and multidrug resistance genes, respectively.\n\n\nCONCLUSIONS\nThe genome sequence of A. baumannii L13 reported here will benefit the comparative analysis of the genus Acinetobacter, and promote further understanding of the specific genomic feature in terms of multidrug resistance in A. baumannii.