Physicochemical properties, bond strength and dual-species biofilm inhibition effect of dental resin composites with branched silicone methacrylate.

Abstract

Dental resin composites (DRCs) with 15\xa0wt% (EC-15%) and 20\xa0wt% (EC-20%) synthesized branched silicone methacrylate (BSM) in resin matrix have showed anti-adhesion effect against Streptococcus mutans. With the aim to evaluate the BSM containing DRCs further, water sorption (WS), solubility (SL), mechanical properties before and after water immersion, anti-adhesion effect against dual-species, bonding strength to adhesive treated dentin, and cytotoxicity of BSM containing DRCs were investigated. DRC without BSM was used as control. The WS and SL were obtained until the mass variation of composite in distilled water kept stable. Three-point bending test was used to evaluate flexural strength (FS) and modulus (FM) of composite before and after water immersion. Mixture of Streptococcus mutans and Lactobacillus acidophilus was used to study the anti-adhesion effect against dual-species. Bonding strength of composite to adhesive treated dentin was measured through macro-shear test. Extract of composite was used to evaluate its cytotoxicity effect on L-929 mouse fibroblasts, and cell viability was obtained by MTT assay. The results showed that EC-15% and EC-20% had similar WS and SL as control (p\xa0<\xa00.05); After water immersion, FS and FM of all composites decreased (p\xa0<\xa00.05), but there was no significant difference in value of FS and FM between different groups (p\xa0>\xa00.05); More bacteria were recovered from the surface of control than those from the surface of EC-15% and EC-20% (p\xa0<\xa00.05); Extract of EC-15% was less cytotoxic (higher cell viability) than those EC-20% and control (p\xa0<\xa00.05). All of results revealed that incorporation of 15\xa0wt% or 20\xa0wt% BSM in resin matrix could endow DRC with inhibition effect on dual-species biofilm formation without impairing physiochemical properties, bonding strength to adhesive treated dentin, and cytotoxicity of DRC.