An investigation of the effects of amniotic fluid on experimental ischemia/reperfusion damage in rat testes.

Abstract

INTRODUCTION\nVarious agents have been tested as preventive treatments for ischemia/reperfusion (IR) damage. In this study, we have investigated for the first time in the literature the efficacy of injection of amniomax (AMX) into testicular parenchyma, which is a commercial medium of rat amniotic fluid, in preventing testicular IR damage related to testicular torsion.\n\n\nOBJECTIVE\nThis study aims to evaluate whether or not amniomax has an effect on experimental IR damage in rat testes using biochemical and histopathological methods based on data in the literature. Even if testicular torsion is repaired surgically in early term injury because of de IR damage still occurs. Is it possible to reduce the ischemia reperfusion injury with amniotic fluid and increase the success of treatment?\n\n\nSTUDY DESIGN\n40 male Wistar albino rats were included. Four groups were formed with 10 rats in each group: Sham, Ischemia/Reperfusion (IR), injection 1\xa0min before detorsion (AMX-BD), injection 1\xa0min after detorsion (AMX-AD). Total Oxidant Status (TOS) and Oxidative Stress Index (OSI) were computed for oxidative stress, and Total Antioxidant Status (TAS) levels were computed for the antioxidant system, for both serum and tissue. Necrosis and microcalcification levels were assessed in the evaluation of testicular histology. P\xa0<\xa00.05 was considered statistically significant.\n\n\nRESULTS\nAMX-AD group has low necrosis degree than IR, Mean serum and tissue levels of TAS, TOS, and OSI parameters were respectively determined as; for TAS: 0.64\xa0±\xa00.11 and 0.96\xa0±\xa00.25\xa0mmol Trolox Equivalent/L; for TOS: 6.71\xa0±\xa00.87 and 9.40\xa0±\xa01.03\xa0μmol\xa0H2O2 equivalent/L; for OSI: 11.94\xa0±\xa03.74 and 10.70\xa0±\xa04.23 arbitrary unit.\n\n\nDISCUSSION\nOur study has investigated for the first time in the literature the efficacy of amniotic fluid in preventing testicular IR damage, and used amniomax (AMX) for this purpose. The limitation of our study may be the small number of rat in the groups.\n\n\nCONCLUSION\nWe think an injection after detorsion is more favorable considering that the AMX-AD group demonstrated significantly lower levels of TOS in serum and tissue and OSI in serum, and significantly higher serum levels of TAS compared to the AMX-BD group, as well as the fact that the morphological protection effect was only observed for injections performed immediately after detorsion.