Journal of structural biology | 2021
Identification of incorrectly oriented particles in Cryo-EM Single Particle Analysis.
Abstract
The quality of a 3D map produced by the single-particle analysis method is highly dependent on an accurate assignment of orientations to the many experimental images. However, the problem s complexity implies the presence of several local minima in the optimized goal functions. Consequently, validation methods to confirm the angular assignment are very useful to yield higher-resolution 3D maps. In this work, we present a graph-signal-processing-based methodology that analyzes the correlation landscape as a function of the orientation, an approach allowing the estimation of the assigned orientations reliability. Using this method, we may identify low-reliability images that probably incorrectly contribute to the final 3D reconstruction.