The Journal of surgical research | 2019
Adventitial Collagen Cross-Link Reduces Intimal Hyperplasia in a Rabbit Arteriovenous Graft Model.
Abstract
BACKGROUND\nIntimal hyperplasia (IH) is the initial lesion of vein graft failure after coronary artery bypass grafting. The weak venous wall is likely one of the primary reasons for IH after exposure to the arterial environment. We investigate whether adventitial collagen cross-link by glutaraldehyde (GA) reinforces the venous wall and then reduces IH.\n\n\nMATERIALS AND METHODS\nAdventitial collagen cross-link by 0.3% GA was performed on the rabbit jugular veins. The degree of cross-link was accessed by tensile test. The jugular vein with or without cross-link was implanted into the carotid artery of rabbit. Vein dilatation at the immediate anastomosis and pathological remodeling of vein graft after 4\xa0wk was assessed.\n\n\nRESULTS\nTensile test indicated that the mechanical property of 3-min cross-linked veins more closely resembled that of the carotid artery. In rabbit arteriovenous graft models, 3-min adventitial collagen cross-link limited overdistension (diameter: 3.24\xa0mm versus 4.65\xa0mm, P\xa0<\xa00.01) at the immediate anastomosis and reduced IH (intima thickness: 78.83\xa0μm versus 140.19\xa0μm, P\xa0<\xa00.01) of vein grafts 4\xa0wk after implantation in the cross-link group as compared with the graft group (without cross-link). Compared with the cross-link group, the expression of proliferating cell nuclear antigen and vascular cell adhesion molecule-1 increased significantly at both the mRNA and protein levels within the graft group (P\xa0<\xa00.01), but the expression of smooth muscle-22α decreased significantly (P\xa0<\xa00.01).\n\n\nCONCLUSIONS\nAdventitial collagen cross-link by GA increased the vessel stiffness and remarkably reduced IH in a rabbit arteriovenous graft model.