How pollution effects the immune systems of invertebrate organisms (Mytilus galloprovincialis Lamark, 1819).

Abstract

Hemocytes are the main immunologic parameters for invertebrate organisms as a response to diseases and pollutions. This study was aimed to reveal the relations between pollution and the total and differential hemocytes numbers. The gulf of İzmir was selected as location for sampling due to its contaminated by different anthropogenic pollutants mainly industrial effects. The effects of the pollutants were investigated on the mussels that have been collected from eight stations in the Izmir Bay (1- Inciralti, 2- Göztepe 3-Konak-4- Pasaport 5-Alsancak, 6-Karşıyaka, 7-Bostanlı), which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey) and 8-Foça, (an anthropogenically industrialized and shipping -impacted area). The pollution effects in the bay were determined on hemolymphs by biomarkers such as total hemocyte counts (THCs) and differential hemocyte counts (DHCs). Microscopic examinations have shown the statistical differences at THCs and DHCs. The highest total hemocyte number was determined in the station 7 (Bostanlı) and the lowest in the station 4 (Pasaport) (P\xa0<\xa00.05). The number of total hemocyte counts were not differed significantly in the Stations among 2 (Göztepe), 5 (Alsancak) and 6 (Karşıyaka), and between Stations 8 (Foça) and 1 (Inciraltı) (P\xa0>\xa00.05). In addition, there have been significant differences in subpopulation according to differential hemocyte numbers. The results indicate that the station 8 (reference site-Foça) has the highest agranulocyte number while station 7 (Bostanlı) and the station 1 (İnciraltı) have the lowest agranulocyte numbers (P\xa0<\xa00.05). As for the basophilic hemocyte numbers, this situation was reversed highest number in station 1 (Inciraltı) and 7 (Bostanlı) and lowest in the station 8 (Foça) (p\xa0<\xa00.05). The station 4 (Pasaport) has the highest eosinophilic hemocyte number while station 8 (Foça) has the lowest. We concluded that differential hemocytes counts are more sensitive as biomarker with the highest numbers of agranulocytes and lowest numbers of eosinophilic and basophilic hemocytes in reference site.