Antibacterial activity absence UV irradiation of Ag, TiO2 and ZnO NPs prepared by sparking method

Abstract

Abstract Silver (Ag) Titanium dioxide (TiO2) and Zinc Oxide (ZnO) nanoparticles (NPs) successfully prepared into distilled water using sparking method. An antibacterial activity against Escherichia coli (E. coli) was typically studied by applying the spread plate test. The bacterial activity was estimated by a relative number of viable cells which form colonies on the nutrient agar plates. From the results, the particle sizes linearly decreased with increasing of the melting temperature of each metal tips. Moreover, the deposition rate of NPs in the water depends on the sparking time and melting temperature. The antibacterial activity absence UV irradiation of Ag and TiO2 completely inhibited after 1 h. Although in the dark, TiO2 can be damage and disorganize the cell membrane due to the nanoparticles in the sizes less than 20 nm can diffuse into the cell membrane of E. coli. The cells were then deformed and intracellular structures were disorganized.