Journal of microbiological methods | 2019
Filtering maxRatio results with machine learning models increases quantitative PCR accuracy over the fit point method.
Abstract
With qPCR reaching thousands of reactions per run, assay validation needs automation. We applied support vector machine to qPCR analysis and we could identify reactions with 100% accuracy, dispensing them from further validation. We achieved a greatly reduced workload that could improve high-throughput qPCR analysis.