Green and non-leaching anti-bacterial and cytocompatible coating with build-in quaternary ammonium salt derived from methoxysilane functionalized soybean oil.

Abstract

Preparation and evaluation of non-leaching antimicrobial polymeric coatings, derived from soybean oil, against bacterial contamination of some metallic biomedical devices are described in this work. Cyclic carbonate derivative of soybean oil (CSBO) was subjected to the reaction with 3-aminopropyl trimethoxysilane to produce a methoxysilane functional soybean oil (Si-SBU) with urethane linkage. A quaternary ammonium salt containing fatty amide molecule with reactive siloxane moiety (Si-SBQ) was also synthesized from soybean oil. The antimicrobial coatings were prepared through sol-gel hydrolysis/condensation reactions of Si-SBU mixed with different concentrations of Si-SBQ under atmospheric moisture condition. The coatings demonstrated very good hardness (202-263\u202fs-1) and superior adhesion strength (2.25-2.95\u202fMPa) to aluminum plate as a model metallic substrate. The biological activities of these coatings were investigated against both fibroblast cells and microorganism strains of Meticillin-resistant S. aureus, P. aeruginosa, and C. albicans. The coatings showed excellent cytocompatibility as they could support growth and proliferation of fibroblast cells (95-98% viability) on their surface. Meanwhile, they showed a high bactericidal activity against all the selected microorganisms.