Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients.

Abstract

OBJECTIVE\nThe aim of the present study was to investigate the effects of prebiotics (containing fructooligosaccharides, xylooligosaccharides, polydextrose, and resistant dextrin) intake on immune function and intestinal microbiota structure in perioperative patients with colorectal cancer (CRC).\n\n\nMETHODS\nA randomized, double-blind, no-treatment parallel control clinical trial involving 140 perioperative patients (90 men and 50 women, aged 40-75 y) with CRC was performed. Patients were randomly divided into two groups: an intervention group (prebiotic group, n\u202f=\u202f70) that received prebiotic supplementation of 30 g/d for 7 d, and a control group (non-prebiotic group, n\u202f=\u202f70) that received no prebiotic supplementation. The nutritional and immunologic indices were evaluated for both groups before and after operation and analyzed against baseline values. Moreover, fecal samples were collected from 40 patients randomly chosen from the two groups to study intestinal microbiota, which was analyzed by sequencing the V3-V4 region of 16S ribosomal DNA using the Illumina (San Diego, CA) MiSeq (PE 2\u202f×\u202f300 bp) platform.\n\n\nRESULTS\nOral intake of prebiotics produced significant effects on immunologic indices in both the preoperative and postoperative periods, but the patterns of effects were different. In the preoperative period, prebiotics increased serum levels of immunoglobulin G (IgG; P\u202f=\u202f0.02), IgM (P\u202f=\u202f0.00), and transferrin (P\u202f=\u202f0.027; all P < 0.05). In the postoperative period, enhanced levels of IgG (P\u202f=\u202f0.003), IgA (P\u202f=\u202f0.007), suppressor/cytotoxic T cells (CD3+CD8+; P\u202f=\u202f0.043), and total B lymphocytes (CD19+; P\u202f=\u202f0.012) were identified in the prebiotic group (all P < 0.05). The differences in the intestinal microbiota at the phylum level were not statistically significant between the intervention and control groups (P > 0.05). At the genus level, prebiotics increased the abundance of Bifidobacterium (P\u202f=\u202f0.017) and Enterococcus (P\u202f=\u202f0.02; both P < 0.05) but decreased the abundance of Bacteroides (P\u202f=\u202f0.04) in the preoperative period (all P < 0.05). In the postoperative period, the abundance of Bacteroides (P\u202f=\u202f0.04) was decreased, but the abundance of Enterococcus (P\u202f=\u202f0.00), Bacillus (P\u202f=\u202f0.01), Lactococcus (P\u202f=\u202f0.00), and Streptococcus (P\u202f=\u202f0.037) increased in the non-prebiotic group (all P < 0.05); however, no significant change was identified in the abundance of Enterococcus (P\u202f=\u202f0.56), Lactococcus (P\u202f=\u202f0.07), and Streptococcus (P\u202f=\u202f0.56) as a result of prebiotic intervention in this period (all P > 0.05). The abundance of Escherichia-Shigella was increased after prebiotic intake in the postoperative period (P\u202f=\u202f0.014, P < 0.05). There was a notable trend of decline in the abundance of intestinal microbiota from preoperative to postoperative in the non-prebiotic group.\n\n\nCONCLUSIONS\nPrebiotic intake is recommended to improve serum immunologic indicators in patients with CRC 7 d before operation. Prebiotics improved the abundance of four commensal microbiota containing opportunistic pathogens in patients with CRC. Surgical stress decreased the abundance of most intestinal microbiota in the intestinal tract but increased the abundance of some opportunistic pathogens and commensal microbiota. Bacteroides is a relevant bacterial species for further research on the mechanism of prebiotics.