Transcriptome analysis and response of three important detoxifying enzymes to Serratia marcescens Bizio (SM1) in Hyphantria cunea (Drury) (Lepidoptera: Noctuidae).

Abstract

Hyphantria cunea (Drury) (Lepidoptera: Noctuidae) is a main pest of forest trees. In this study, the effects of Serratia marcescens Bizio (SM1) infection on the transcriptome of H. cunea were studied. The expression of 1068 unigenes in the transcriptome of H. cunea infected by S. marcescens was markedly different from that in the control of H. cunea; 474 genes were upregulated, and 594 genes were downregulated in the former. Among them, 8 cytochrome P450s (CYPs), 5 uridine diphosphate-glycosyltransferases (UGTs) and 3 glutathione S-transferases (GSTs) were significantly differentially expressed. Pathway enrichment analysis indicated that these differentially expressed detoxification enzyme genes were mainly involved in the drug metabolism pathway, glutathione metabolism pathway and ABC transporter pathway. Interestingly, we found that five UGTs were related to oestradiol metabolism in the steroid hormone biosynthesis pathway. Furthermore, the real-time fluorescent quantitative PCR results showed that SM1 could induce the expression of CYPs and UGTs, but inhibit the expression of GSTs. This research will identify the response of important detoxification enzymes to S. marcescens, which will provide a theoretical foundation for the development of new immunosuppressants for H. cunea control. Furthermore, H. cunea was performed transcriptome sequencing to explore the key metabolic pathways, signalling pathways and genes affected by S. marcescens, which will clarify the mechanisms of S. marcescens infection of H. cunea. In addition, this study also explored the relationship between H. cunea and S. marcescens, which will provide a theoretical basis for the biological control of H. cunea by using S. marcescens.