A novel borate fluorescent probe for rapid selective intracellular peroxynitrite imaging.

Abstract

Peroxynitrite (ONOO-) is one of the species of reactive nitrogen (RNS), which plays an important role in antibacterial activity and signal transduction and other physiological and pathological processes. In this paper, based on the benzyl borate group, a new fluorescent probe capable of detecting ONOO- with high selectivity and sensitivity is designed, and the possible mechanism of the interaction between probe and ONOO- is proposed. The probe shows high fluorescence response to ONOO- in a wide pH range (7.0-11.5). Moreover, the probe exhibit good permeability, and the content of ONOO- in cancer cells and normal cells was successfully monitored.