A bifunctional fluorescent probe for simultaneous detection of GSH and H2Sn (n\u202f>\u202f1) from different channels with long-wavelength emission.

Abstract

In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (H2Sn, n\u202f>\u202f1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (λex/em\u202f=\u202f430/530\u202fnm). In the presence of H2Sn, the probe exhibited a significant fluorescence enhancement in red channel (λex/em\u202f=\u202f560/680\u202fnm). We also demonstrated that this probe was suitable to quantitatively detect GSH and H2Sn with low detection limits (0.12\u202fμM for GSH, 0.19\u202fμM for H2Sn). Furthermore, DCM-Cou-SePh can be used for sensing endogenous GSH and H2Sn in living cells by dual-color fluorescence imaging.