A colorimetric sensor based on Glutathione-AgNPs as peroxidase mimetics for the sensitive detection of Thiamine (Vitamin B1).

Abstract

A label-free sensing strategy based on the enzyme-mimicking property of Glutathione-Ag nanoparticles (GSH-AgNPs) was demonstrated for colorimetric detection of vitamin B1 (VB1). Firstly, obvious blue color accompanied with an absorption peak at 652\xa0nm was observed due to the high peroxidase-like activity of GSH-AgNPs towards 3,3 ,5,5 -tetramethylbenzidine (TMB). Then, in the presence of VB1, the mimetic activity of GSH-AgNPs could be strongly restrained, evidenced as a promiment colorimetric change to colorless, which can be used to achieve the visualization detection VB1. Linear relationship between absorbance response and VB1 concentration from 0 to 0.2\xa0µM were obtained. The detection limit was calculated as low as 40\xa0nM. The inhibition reasons were thoroughly discussed. Considering the advantages of rapid response, easy procedure and high selectivity, the proposed method possesses potential application in environment and biological analysis for VB1 detection.