Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus.

Abstract

The endocrine disrupting effect of pesticides is considered to be an important factor in the decline of reptile populations. The large-scale application of neonicotinoids in the environment poses a potential threat to small farmland lizards Eremias argus. In this study, we evaluated the disruption effects of thiamethoxam and its metabolite clothianidin on the endocrine disruption of Eremias argus during 28\u202fd exposure. Thiamethoxam and clothianidin could accumulate in the testis and ovary. Adequate blood exchange was the main cause of thiamethoxam and clothianidin accumulation in the gonads. The production of clothianidin aggravated the effect of endocrine disruption to lizards. Thiamethoxam/clothianidin exhibited two distinct ways of interfering with the endocrine disruption of the male and female lizards. Thiamethoxam/clothianidin significantly up-regulated the expression of cyp17 and cyp19 genes in the testis, which ultimately led to a significant decrease in testosterone levels and a significant increase in the 17-estradiol concentrations in plasma. The expression of the estrogen receptor gene in the liver was also significantly increased in male lizards. The significant declines in testosterone and prostaglandin D2 levels in the plasma indicated that thiamethoxam and clothianidin could cause androgen deficiency in male lizards. Meanwhile, in female lizards, thiamethoxam/clothianidin increased the expression of hsd17β gene in the ovary, causing an increase in testosterone levels in the plasma and an up-regulation of androgen receptor expression in the liver. The effects of thiamethoxam and clothianidin on male lizards were more pronounced. This study verified the possible endocrine disrupting effects of neonicotinoids and provided a new perspective for the study of global recession of reptiles.