Insight into dynamics and bioavailability of antibiotics in paddy soils by in situ soil moisture sampler.

Abstract

Plant-soil systems have complex regulatory mechanisms for xenobiotics uptake by plant, and these chemicals in soil pore water (SPW) are regarded as the bioavailable fraction. To date, little is known about the role of SPW in regard to the bioavailability of antibiotics for plant. In this study, in situ soil moisture sampler (SMS) was adopted to collect SPW from four paddy soils without disrupting the rhizosphere zone to evaluate antibiotic uptake in rice. The results show that SMS is applicable for antibiotics that have small molecular sizes and Log Kow values, e.g., sulfadiazine (SDZ), sulfamethoxazole (SMZ), trimethoprim (TRM), and florfenicol (FLR). However, SMS performance was not feasible for large size and medium hydrophobic clarithromycin (CLR). Antibiotics in SPW demonstrated differences among chemicals and soils. Relatively higher levels of SDZ, SMZ, and FLR were observed in SPW than TRM, and neutral Panjin soil had the highest levels of antibiotics in SPW among four soils. The levels of antibiotics in SPW were negatively correlated with their soil partition parameter, Kd. Rapid decreases of SMZ, FLR, and SDZ in the SPW were consistent with their low residues in the final soils. All antibiotics were detectable in rice roots, followed by low detection levels in a few shoot samples, while no antibiotics were detectable in the grains of four soils. Relatively higher levels of SDZ, SMZ and FLR were observed in the roots of Panjin soil, consistent with their levels in SPW and Kd values. Furthermore, CLR and TRM were observed to have higher levels in roots, which was regarded as a consequence of their relatively longer persistence. Our study indicates that SMS is an applicable technique for in situ sampling of SPW, and level of antibiotics in SPW can work as a useful indicator to explore their bioavailability to plants.