Novel antifouling polymer with self-cleaning efficiency as surface coating for protein analysis by electrophoresis.

Abstract

The non-specific adsorption of protein has caused many problems in the application of materials. In this paper, a tri-block copolymer PEO-PNIPAAm-PSPMAP with double effects were obtained via atom transfer radical copolymerization (ATRP). The double-effect copolymer is covalently bonded to the hydrophobic material through a photosensitizer to achieve surface modification and applied to analytical chemistry. Sufficient hydratable groups (for instance, ether bonds, amide groups, and sulfonic acid groups) in the copolymer provides a basis for the anti-protein adsorption. At the same time, the interaction of the hydrophilic group and isopropyl group with temperature changes provides the possibility of elastic self-cleaning of the material, which is instrumental in extending the circulate lifetime of materials. Therefore, it is an environmentally friendly coating material. Besides, the effective antifouling performance and elastic self-cleaning function of the coating have been confirmed by the dynamic adsorption experiment of a fluorescent protein. The coating is used in capillary electrophoresis (CE), and its excellent protein separation spectrum verifies the practicality of the coating.