TopoStats – A program for automated tracing of biomolecules from AFM images

Abstract

Highlights • TopoStats is a Python toolkit for automated AFM data processing.• TopoStats takes raw data as input, and outputs processed data and statistical information without user input.• TopoStats can accurately trace linear and circular biomolecules and provide conformational analysis.• We applied TopoStats to analyse DNA minicircles, origami and pore-forming proteins.• TopoStats can be used on both ideal and contaminated samples.