Resveratrol inclusion alleviated high dietary carbohydrate induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides.

Abstract

Excessive hepatic glycogen accumulation commonly impairs hepatocytes function, and further produces negative effects on growth and health status of carnivorous fish. A 9-week feeding trial was conducted to explore the potential regulation of resveratrol on high carbohydrate induced glycogen deposition and immune response of largemouth bass. Results showed that high dietary carbohydrate (10% inclusion of starch) led to hepatic glycogen accumulation and postprandial hyperglycemia compared to the diet with 5% starch, which was both alleviated with the inclusion of resveratrol. The use of resveratrol promoted the expression of sirtuin 1, which was down-regulated by high dietary carbohydrate. Meanwhile, resveratrol inclusion promoted the expression of genes involved in insulin pathway and glycolysis, and inhibited the expression of gluconeogenesis related genes. Additionally, high dietary carbohydrate significantly reduced lysozyme content but increased complement C4 content, which were both reversed with resveratrol supplementation. Meanwhile, resveratrol inclusion inhibited the expression of pro-inflammatory cytokines, but promoted anti-inflammatory cytokines expression, compare to the high carbohydrate treatment. In conclusion, resveratrol inclusion was beneficial in alleviating high dietary carbohydrate induced glycogen accumulation and immune response in largemouth bass.