Introducing the Pacific Northwest Center for Cryo-EM

Abstract

PNCC Capabilities [5] • 120kV T12 Spirit – negative stain screening and training *availability may be limited • 200kV Talos Arctica – screening, training, Micro-ED, single-particle data collection o CETA, Gatan K2 DED • 300kV Titan Krios – single-particle or tomography data collection and training * available June 2019 o CETA, Gatan K3 DED post GIF, Falcon 3 DED, Volta Phase Plate • 300kV Titan Krios – single-particle data collection and training * available June 2019 o CETA, stand-alone Gatan K3 and Falcon 3 DEDs • 300kV Titan Krios – single-particle data collection * available June 2019 o CETA, Falcon 3 DED • Low Humidity WetLab Sample Optimization and Preparation o Vitrobot Marck IV, Leica GP2, manual plunge-freezer, Centrifuge, Ultracentrifuge, FPLC, HPLC, DLS