New Antidiabetic and Free-Radical Scavenging Potentialof Strictosamide in Sarcocephalus pobeguinii Ground Bark Extract via Effect-Directed Analysis

Abstract

The active principle of most traditional medicines is not known, as for Sarcocephalus pobeguinii Hua ex Pellegr. bark extract. It is used as an antidiabetic drug in Gabonese folk medicine. Diabetes mellitus is increasing globally, and products from natural sources are gaining interest as a remedy. As the active antidiabetic compounds have not been characterized so far, a mobile phase for high-performance thin-layer chromatography (HPTLC) was newly developed to target single active compounds in the unpolar ground bark extract of S. pobeguinii by effect-directed analysis (EDA). One natural compound zone showed multipotent activities by effect-directed detection, that is, antidiabetic, cholinesterase inhibiting, and antioxidative activities. Its characterization was performed via online elution of the active substance zone from the HPTLC layer into the high-resolution mass spectrometry (HRMS) system. Important parts of the structure were assigned by MS/MS experiments and led to the preliminary assignment of ...