Latent Redox Reporter of 4-Methoxyphenol as Electrochemical Signal Proxy for Real-Time Profiling of Endogenous H2O2 in Living Cells.

Abstract

Hydrogen peroxide (H2O2) plays a persuasive role in the human defense mechanism cell physiology. Developing an efficient assay platform; a high sensitive tracking and quantification of H2O2 in a physiological system is of great importance are crucial to understanding the neoplastic changes and/or redox homeostasis of cells. In this study, a novel strategy, new-of-a-kind turn-on latent electrochemical redox probe coupled electrocatalytic signal amplification strategy is proposed., A custom made readily available turn-on latent electrochemical probe 4-methoxyphenylboronic acid pinacol ester (4-MPBP) was have designed for selective detection of endogenous H2O2 endogenous in live cells. The designed electrochemical probe composed of a latent electrochemical reporter unit (4-Methoxy phenol, 4-MP) linked with bearing a target recognition unit (boronic acid pinacol ester) for H2O2 sensing. The selective analyte-triggered signal chemical transformation release free electrochemical reporter 4-MP. mechanism towards The amount of H2O2 were was evaluated electrochemically at glassy carbon electrode (GCE) in with a broad dynamic detection range of 0.5 µM to 1mM. An amplified signal response of released 4-MP from 4-MPBP to build a high sensitive assay tool was have been proficiently achieved by via replacing the GCE transducer electrode with Polydopamine@carbonnanotube-Molebtinumdisulfie hybrid modified GCE (PDA@CNT-MoS2/GCE) with as it delivered an exceptional dynamic detection range of 0.01 µM to 100 µM. The innovative blend of electrochemical molecular probe strategy, with electrocatalytic signal amplification technique has delivered outstanding assay performance at trace level sensing of H2O2. Next, we set up a platform for real-time in-vivo monitoring of endogenously produced H2O2 in Caco-2 cells, and MCF-7 cells through spermine-polyamine analogue, and phorbol 12-myristate 13-acetate (PMA) induction in SSAT/PAO gene and protein kinase C (PKC), respectively. As expected, the 4-MPBP latent probe coupled electrocatalytic signal amplification strategy delivered outstanding performance towards An efficient in-situ H2O2 release, and tracking, over time. and quantification prowess was observed electrochemically through 4-MPBP latent probe coupled electrocatalytic signal amplification approach.