Synthesis and Application of CeO2/SnS2 Heterostructures as Highly-Efficient Coreaction Accelerator in Luminol-Dissolved O2 System for Ultrasensitive Biomarkers Immunoassay.

Abstract

Electrocheluminescence immunoassay amplified by coreaction accelerators has experienced major breakthroughs in ultrasensitive detection of biomarkers. Herein, CeO2/SnS2 heterostructures were synthesized and applied as novel coreaction accelerator to enhance the ECL efficiency of luminol-dissolved O2 system for the first time. Benefiting from the well-matched lattice spacing, ultrafine CeO2 nanoparticles (NPs) were in situ grew on layered SnS2 nanosheets (NSs) with improved dispersion. CeO2/SnS2 as a electroactive substrate can remarkably accelerate the generation of abundant superoxide anion radicals (O2•-) to react with luminol anion radical (L•-), achieving about 2-fold stronger ECL intensity than pure CeO2 NPs. To avoid harsh chemical synthesis of conventional ECL labels and simplify antibody conjugation process, ferritin (Ft) was served as a natural nanocarrier to immobilize luminol molecules (Lum@Ft) via one-step linkage, whose protein nanocage can easily connect with detection-antibody. Moreover, a robust site-oriented immobilization strategy using HWRGWVC heptapeptide as specific capturer was further adopted to maintain the bioactivity of capture-antibody on the amine-functionalized CeO2/SnS2 surface, which promoted the incubation efficiency markedly. On account of this advanced sensing strategy, a brand-new biosensor was constructed for the accurate detection of heart failure biomarkers, which performed favorable linearity in the range of 0.0001 - 50 ng/mL and achieved the detection limit of 36 fg/mL.