Association of apoptosis genes in PDCD1 but not PDCD1LG2, FAS, and FASLG with pediatric idiopathic uveitis in Han Chinese

Abstract

Background Previous studies have shown that aberrant T lymphocyte apoptosis is involved in the pathogenesis of uveitis. Genetic variants of apoptotic pathway-related factors (including PDCD1, PDCD1LG2, FAS, and FASLG) may affect apoptosis and in turn predict susceptibility to autoimmune disease. This has not yet been studied in pediatric idiopathic uveitis (PIU) and juvenile idiopathic arthritis (JIA)-associated uveitis and was therefore the subject of the study presented here. Methods Fourteen single-nucleotide polymorphisms (SNPs) of several apoptosis-related pathway genes were analyzed in 1238 PIU patients, 128 JIA-associated uveitis patients and 1114 healthy controls using the iPLEX Gold Assay and MassARRAY platform. Results A lower frequency of the PDCD1/rs6710479 CC genotype in PIU patients was found when compared to controls (Pc\u2009=\u20093.42\u2009×\u200910 −3 ). A higher frequency of the PDCD1/rs7421861 A allele (Pc\u2009=\u20094.85\u2009×\u200910 −3 ) was observed in PIU patients as compared with controls. Stratification analysis showed a positive association of band keratopathy with the PDCD1/rs7565639 CT genotype (Pc\u2009=\u20091.05\u2009×\u200910 −2 ) and a negative association of this parameter with the PDCD1/rs7565639 C allele (Pc\u2009=\u20093.76\u2009×\u200910 −2 ). Conclusions This study revealed that rs6710479 and rs7421861 in the PDCD1 gene confer susceptibility to PIU in Han Chinese. A stratified analysis showed that PDCD1/rs7565639 is associated with band keratopathy in PIU patients.