Signal Transduction and Targeted Therapy | 2021
Attenuating innate immunity and facilitating β-coronavirus infection by NSP1 of SARS-CoV-2 through specific redistributing hnRNP A2/B1 cellular localization
Abstract
Dear Editor, Evidence shows the NSP1’s crucial roles of the β-coronavirus SARS-CoV-2 in promoting cellular mRNA degradation, inhibiting host cell translation, innate immunity, and inducing inflammatory cytokine storm in the pathogenesis of COVID-19. More interestingly, NSP1 deletion in infectious clones prevents virus infection. However, little is known how NSP1 interacts with host factors to disrupt the host’s innate immunity for facilitating virus infection and reproduction. As a (+) ssRNA virus, SARS-CoV-2 completes its life cycle in the cytosol; viral RNA processing is the key for controlling and regulating the virus reproduction and pathogenesis. The ribonucleoproteins hnRNPs are the main factors responsible for RNA processing, including RNA splicing, maturation, decay, and translation, and even innate immunity in some cases. To facilitate viral RNA processing, hnRNPs must redistribute from nucleus to the cytoplasm. We expressed SARS-CoV-2 encoded proteins NSP1, NSP2, NSP5, ORF8, and NSP12 (FLAG-tagged at the C-terminal) in Rhabdomyosarcoma cells and examined their effects on hnRNPs’ subcellular distribution. After immunostaining with specific antibodies against hnRNP A2/B1 (also called hnRNP A2 or hnRNP B1), hnRNP D, hnRNP K, and hnRNP L proteins, surprisingly, we observed that only NSP1 specifically induced the hnRNP A2/B1 redistribution from the nucleus to the cytoplasm (Fig. 1a) but had no effect on its subfamily member hnRNP A1 (Fig. 1b). All other viral proteins (NSP2, NSP5, ORF8, and NSP12) failed to redistribute hnRNP A2/B1 and the other tested hnRNPs (Supplementary Fig. 1a–d). We further examined the effects of SARS-CoV-2 proteins on hnRNP A2/B1 protein levels. As shown in Supplementary Fig. 3b, NSP14, NSP15, and ORF8 only slightly decreased the protein level of hnRNP A2/B1, while the other NSPs (NSP1, NSP2, NSP3, NSP4, NSP5, NSP9, NSP12, and NSP13) had no obvious effects on hnRNP A2/B1 protein level in HEK293T cells. Recent studies show that NSP1 blocks the initiation of the host mRNA translation, drives mRNAs into the decay pathway, and may impair host immunity and stimulate an inflammatory response. We thus investigated its effects on the host cell’s innate immunity and inflammatory cytokine expression, the main death causes of COVID-19 patients. Interestingly, NSP1 decreased the mRNA level of IFNβ measured by RT-qPCR assay (Fig. 1c, d). We knocked down hnRNP A2/B1 by specific siRNAs, leading to an increased mRNA level of IFNß (Fig. 1e). More importantly, the decreased IFNß mRNA level in NSP1-expressing cells was able to completely restore by silencing hnRNP A2/B1 expression (Fig. 1e). Surprisingly, the mRNA level of major inflammatory factors (e.g., TNFα and IL-6) was not affected by either hnRNP A2/B1 knockdown or the forced NSP1 expression (Fig. 1e). We then conducted two more experiments to examine whether NSP1 affects the gene expression of type I interferons’ downstream antiviral effectors—interferon-stimulated genes (ISGs). (1) With ectopic NSP1 expression, we observed that the mRNA level of ISG56, MX1, RNAase L, and ISG20 was significantly decreased (Fig. 1c, d); (2) We treated HEK 293T cells with IFNα (1000 U/ml) for 30 min and examined the phosphorylation status of STAT1 and STAT2. As shown in Fig. 1f, NSP1 decreased STAT1/2 phosphorylation (Fig. 1f); and STAT2 phosphorylation was significantly boosted by knockdown of hnRNP A2 but not hnRNP A1 upon IFNα stimulation (Fig. 1g). We further performed co-immunoprecipitation assay to determine whether hnRNP A2/B1 could interact with NSP1. We showed that NSP1 directly bonds hnRNP A2/B1 (Fig. 1i, j), implying that SARS-CoV-2 hijacks host hnRNP A2/B1 protein redistribution through direct binding with NSP1 to impair host innate immunity to facilitate SARS-CoV-2 infection. Targeting the host factor displays particular antiviral advantages because it would not only avoid the fast viral mutagenesis but also inhibit viral reproduction and infection against a board range of virus species. We aligned the NSP1 sequences of all human pathogenic β-coronavirus species, including SARS-CoV, SARS-CoV2, and closed related animal species, MERS-CoV, HCoV-HKU1, and HCoV-OC43. As shown in Supplementary Fig. 2, the NSP1 sequence of HCoV-OC43 shares the lowest similarity with SARS-CoV-2 as compared with other pathogenic β-coronavirus species. To prove the concept, we knocked down hnRNP A2/B1 in Rhabdomyosarcoma cells and infected them with HCoV-OC43, and measured the intracellular viral RNA level by RT-qPCR. Knockdown of hnRNP A2/B1 significantly suppressed viral replication by decreasing the viral genomic RNA level by 98%, whereas no antiviral effects were observed when its subfamily member hnRNP A1 was knocked down (Fig. 1h), demonstrating that NSP1 specifically hijacks hnRNP A2/B1’s cellular localization to suppress the host cells’ innate immunity for facilitating SARS-CoV2 and other ß-coronaviruses (such as HCoV-OC43) infections. The molecular mechanism on how NSP1 specifically redistributes hnRNP A2/B1 in the cytosol is to be investigated. We noticed that hnRNP A2/B1 is almost localized in the nucleus in normal cells and redistributed in the cytoplasm (Fig. 1a). We observed NSP1 distribution both in the cytoplasm and nucleus in Hela cells, which is consistent with a recent report. To exclude the possibility that the FLAG-tag affects NSP1 function or distribution, we constructed a plasmid to express the natural NSP1 of SARSCoV-2 in HeLa cells. Both NSP1 with/without Flag-tag induced hnRNP A2 redistribution (Supplementary Fig. 1e). Results from our study revealed that NSP1 formed a complex with hnRNP A2/B1 (Fig. 1i, j). Taken together, we propose a dual mechanism for NSP1 hijacking hnRNP A2/B1 cellular redistribution (Fig. 1k). NSP1 may bind newly synthesized hnRNP A2/B1 in the cytoplasm and block its translocation into the nucleus, and the nuclear NSP1 may form a complex with hnRNP A2/B1 and other unknown proteins that drive hnRNP A2/B1 to translocate into the cytoplasm. NSP1 is