A polythiophene-based chemosensor array for Japanese rice wine (sake) tasting

Abstract

We demonstrated Japanese rice wine (sake) tasting using a fluorescent chemosensor array comprising polythiophene-based chemosensors functionalized with pyridinium boronic acid. The chemosensors bound to the components of sake (i.e., glucose and pyruvate) and showed changes in fluorescence as a result. To achieve on-site analysis, the chemosensor microarray was fabricated on a glass chip, for which an image pattern was rapidly recorded by using a CCD camera equipped with color filters. For high throughput and accurate analysis, the collected datasets were analyzed using pattern recognition techniques, and we discriminated between four nondiluted sakes based on the cross-reactive response of the polythiophene-based chemosensors. Moreover, the facile chemosensor sensor array succeeded in predicting unknown concentrations of glucose and pyruvate in diluted sake, which revealed its usability for drinks analysis. A fluorescent microarray comprising polythiophene-based chemosensors functionalized with pyridinium boronic acid has been developed for the detection of the components of sake such as glucose and pyruvate. The sensor microarray was fabricated on a glass chip for on-site detection utilizing imaging analysis and pattern recognition. The selected four nondiluted sake samples were discriminated by the cross-reactive response pattern. Moreover, the facile chemosensor array realized the prediction of unknown concentrations of glucose and pyruvate in the diluted sake, which indicated its usability for drink analysis.