Feeding experiments on Vittina turrita (Mollusca, Gastropoda, Neritidae) reveal tooth contact areas and bent radular shape during foraging

Abstract

The radula is the food gathering and processing structure and one important autapomorphy of the Mollusca. It is composed of a chitinous membrane with small, embedded teeth representing the interface between the organism and its ingesta. In the past, various approaches aimed at connecting the tooth morphologies, which can be highly distinct even within single radulae, to their functionality. However, conclusions from the literature were mainly drawn from analyzing mounted radulae, even though the configuration of the radula during foraging is not necessarily the same as in mounted specimens. Thus, the truly interacting radular parts and teeth, including 3D architecture of this complex structure during foraging were not previously determined. Here we present an experimental approach on individuals of Vittina turrita (Neritidae, Gastropoda), which were fed with algae paste attached to different sandpaper types. By comparing these radulae to radulae from control group, sandpaper-induced tooth wear patterns were identified and both area and volume loss could be quantified. In addition to the exact contact area of each tooth, conclusions about the 3D position of teeth and radular bending during feeding motion could be drawn. Furthermore, hypotheses about specific tooth functions could be put forward. These feeding experiments under controlled conditions were introduced for stylommatophoran gastropods with isodont radulae and are now applied to heterodont and complex radulae, which may provide a good basis for future studies on radula functional morphology.