Impact of rs2414096 polymorphism of CYP19 gene on susceptibility of polycystic ovary syndrome and hyperandrogenism in Kashmiri women

Abstract

Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder in pre-menopausal women having complex pathophysiology. Several candidate genes have been shown to have association with PCOS. CYP19 gene encodes a key steroidogenic enzyme involved in conversion of androgens into estrogens. Previous studies have reported contradictory results with regard to association of SNP rs2414096 in CYP19 gene with PCOS and hyperandrogenism in different ethnic populations. Present study was aimed to investigate the impact of SNP rs2414096 polymorphism of CYP19 gene on susceptibility of PCOS and hyperandrogenism in Kashmiri women. Further we also studied the genotypic-phenotypic association for various clinical and biochemical parameters of this polymorphism. Case control study. 394 PCOS cases diagnosed on the basis of Rotterdam criteria and age matched 306 healthy women. We found a significant differences in genotypic frequency (χ2\u2009=\u200918.91, p\u2009<\u20090.05) as well as allele frequency (OR 0.63, CI 0.51–0.78, χ2\u2009=\u200917.66, p\u2009<\u20090.05) between PCOS women and controls. The genotype–phenotype correlation analysis showed a significant difference in FG score (p\u2009=\u20090.047) and alopecia (p\u2009=\u20090.045) between the three genotypes. Also, the androgen excess markers like DHEAS (p\u2009<\u20090.001), Androstenedione (p\u2009<\u20090.001), Testosterone (p\u2009<\u20090.001) and FAI (p\u2009=\u20090.005) were significantly elevated in GG genotype and showed a significant difference in additive model in PCOS women. rs2414096 polymorphism of CYP19 gene is associated with the risk of PCOS as well as with clinical and biochemical markers of hyperandrogenism, hence suggesting its role in clinical manifestations of PCOS in Kashmiri women.