Biosynthesis of Red Elemental Selenium Protein

Abstract

The red elemental selenoprotein was biosynthesized by active dry yeast in this study. Through a single factor experiment and L9(34)-orthogonal test fermentation condition optimization, the selenium content was 15.72 mg/g under optimal condition. The optimum culture condition was as follows: the sodium selenite concentration (0.8 mg/mL), culture time (36 h), the yeast dosage (5 g), pH (5.5), culture temperature (30°C), and the medium Baume degree (8 °Be). The analysis of amino acid species, content and morphology of the product showed that the red elemental selenoprotein was successfully synthesized.