The Effect of Sugammadex on Time of Sciatic Block by Perineural Bupivacaine in Rats.

Abstract

OBJECTIVES\nWe aimed to investigate the effect of sugammadex on the motor, sensory and deep sensory block in the sciatic nerve created by bupivacaine in rats.\n\n\nMATERIALS AND METHODS\n18 Sprague-Dawley adult male rats treated with unilateral sciatic nerve block by bupivacaine (0.2\u2009ml) were randomly divided into three groups. Control group (Group C, n\u2009=\u20096, 1.5\u2009mL saline) perineural sugammadex group (Group PNS, n\u2009=\u20096, 16\u2009mg/kg) and intraperitoneal sugammadex group (Group IPS, n\u2009=\u20096, 16\u2009mg/kg) Motor, sensory, and deep sensory functions were evaluated every 10\u2009minutes by a blind researcher. 6 tissue samples each belonging to the sciatic nerve, 1.5\u2009cm in length and 0.2\u2009cm in diameter, were taken from paraffin blocks. Sections of 3-4 micrometers were stained with Hematoxylin\u2009+\u2009Eosin, Masson Trichrome dyes and examined under a light microscope.\n\n\nRESULTS\nThere was no statistically significant difference between 3 groups in terms of the time to return to normal motor, sensory and deep sensory function. There was also no significant difference in edema, extracellular matrix, and myelin. Inflammatory cells were seen in all groups, mainly epineurium, epineurium, and perineurium.\n\n\nCONCLUSION\nThere are findings of no histological effects or effects on local block of sugammadex in rats undergoing sciatic nerve block.