Capacitive hyperthermia as an alternative to brachytherapy in DNA damages of human prostate cancer cell line (DU-145)

Abstract

Abstract Purpose: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high-dose rate brachytherapy (BR). Materials and methods: DU-145 cells were cultured as spheroids in 300 micron diameter. Then the following treatments were conducted: (a) EBRT at doses of either 2\u2009Gy or 4\u2009Gy of photon 15\u2009MV, (b) HT for 0, 30, 60, and 90\u2009minutes duration at 43\u2009°C from a 13.56\u2009MHz radiofrequency capacitive heating device (Celsius TCS), (c) BR with Ir-192 seed at doses of either 2\u2009Gy or 5.5\u2009Gy, (d) The mentioned HT followed by EBRT (HT\u2009+\u2009EBRT) and (e) EBRT followed by BR (EBRT\u2009+\u2009BR). Alkaline comet assay was performed to measure tail moment. Results: The induced DNA damages of DU-145 cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; 3.28 and 5.14 times respectively for 30 and 60\u2009minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT\u2009+\u2009EBRT and EBRT\u2009+\u2009BR treatments. Conclusions: This study suggests that about DNA damages of DU-145 cells, HT\u2009+\u2009EBRT could partly be considered as an alternative to EBRT\u2009+\u2009BR.