Topical application of Jatyadi Ghrita and Jatyadi Taila accelerates wound healing in Sprague-Dawley rats: a study in gamma-radiation-induced skin wound model

Abstract

Abstract Purpose Radiation-induced skin wounds/dermatitis can occur due to therapeutic, occupational, or accidental exposure to ionizing radiation. This study investigated the therapeutic efficacy of standardized Ayurvedic formulations [Jatyadi ghrita (JG) and Jatyadi taila (JT)] against 60Co-γ-radiation-induced acute skin wounds in rats. Material and methods Animal’s [Sprague-Dawley rats (200\u2009±\u200920\u2009g)] flanked skin was locally exposed to 45\u2009Gy radiation (R45Gy) in Cobalt-60-teletherapy unit (Bhabhatron) to generate radiation wounds. JG and JT were applied topically twice daily on wounds from day 14 onwards after appearance of moist desquamation and wound healing efficacy was observed for a period of 42\u2009days. Results R45Gy induced significant time dependent changes in rat’s skin with erythema on day 7 followed by dry and moist desquamation. JG and JT application significantly (p\u2009<\u2009.001) reduced skin damage score, wound area (92% and 97% respectively on day 42), and bacterial load, when compared with R45Gy and showed better efficacy than sucralfate and betamethasone (positive controls). Formulations significantly reduced lipid peroxidation and enhanced antioxidant defenses, reduced inflammatory infiltrates and collagen fibers deposition as evident by decreased myeloperoxidase and hydroxyproline levels, and also reduced transforming growth factor-beta 1 (TGF-β1) expression. Further, histology revealed reduced epidermal hyperplasia and dermal thinning with improved densities of hair follicles. Formulations were found to be nontoxic on 28\u2009days application. Conclusions The results demonstrated that JG and JT accelerated wound healing in irradiated skin tissue by faster re-epithelialization; reducing inflammation, collagen fibers deposition, and TGF-β1 expression, indicated their potential human application in countering radiation wounds. Graphical Abstract Therapeutic efficacy of standardized Ayurvedic formulations [Jatyadi ghrita (JG) and Jatyadi taila (JT)] were evaluated against gamma-radiation-induced acute skin wounds in rats. JG and JT when applied topically on irradiated skin tissue accelerated wound healing by faster re-epithelialization, reducing inflammation, collagen fiber deposition, and TGF-β1 expression, indicated their potential human application in countering radiation wounds. Graphical Abstract