Development of an UHPLC-MS/MS method for remifentanil quantification in a small plasma volume

Abstract

Abstract Remifentanil is a short acting opioid currently used in anesthesia and as an analgesic. This paper describes a simple, fast HPLC-MS/MS methodology that allows detection of remifentanil in low volume plasma samples. Acetonitrile protein precipitation is used for sample extraction and clean up. The assay has a lower limit of detection of 0.25\u2009ng/mL and a 3\u2009min run time. Graphical Abstract