Ferula pseudalliacea induces apoptosis in human colorectal cancer HCT-116 cells via mitochondria-dependent pathway

Abstract

Abstract Ferula species have diverse biological functions. This study set out to investigate the anti-proliferative effects of methanolic extract of F. pseudalliacea against human colon cancer HCT-116 cell line. Cytotoxic effects of F. pseudalliacea on HCT-116 cells was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Real-time polymerase chain reaction (PCR) and Western blot were employed to analyze BAX and Bcl2 expression. Cell cycle analysis and apoptosis were conducted using flowcytometry and Annexin V/ propidium iodide (PI) staining. Rhodamine 123 staining and enzyme-linked immunosorbent assay (ELISA) assay were employed to quantify the mitochondria membrane potential (MMP) and caspase 3 activity, respectively. F. pseudalliacea markedly decreased HCT-116 cells viability. The gene and protein expression of BAX were increased, whereas Bcl-2 was decreased in F. pseudalliacea treated cells. F. pseudalliacea induced apoptosis via promotion of cell cycle arrest, caspase 3 activation, and destruction of MMP. These results demonstrate that F. pseudalliacea extract is able to induce apoptosis in HCT-116 cells mainly by activation of the mitochondrial pathway.