Association of methionine synthase (rs1805087), methionine synthase reductase (rs1801394), and methylenetetrahydrofolate dehydrogenase 1 (rs2236225) genetic polymorphisms with recurrent implantation failure.

Abstract

One-carbon metabolism, in which folate plays an essential role, is important for maintaining pregnancy and foetal development. Here, polymorphisms in three genes involved in the methylation of homocysteine were examined: methionine synthase (MTR), methionine synthase reductase (MTRR), and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), each of which is involved in methionine metabolism, a component of the one-carbon metabolism process. This involved a case-control study of 343 Korean women: 118 patients with RIF and 225 controls with at least one live birth and no history of pregnancy loss. The MTHFD1 1958GA genotype was observed less frequently than the MTHFD1 1958GG genotype (IF ≥ 4, p\u2009=\u20090.015) in women with RIF. In addition, the MTRR 66A\u2009>\u2009G polymorphism was associated with increased plasma homocysteine levels (p\u2009=\u20090.019). Based on these results, we propose that the MTRR 66A\u2009>\u2009G and MTHFD1 1958G\u2009>\u2009A polymorphisms are predisposing factors for RIF development. This study is the first to examine a potential association between the MTHFD1 and MTRR polymorphisms and RIF susceptibility in Korean patients.