Combination of HRLC-MS with MPP and GNPS data analysis to identify major constituents of MtSK inhibitory Alpinia galanga extract.

Abstract

Alpinia galanga (L.) Willd. (Zingiberaceae), or galangal, has been previously reported as active against Mycobacterium tuberculosis (TB) in\xa0vitro. The present study assessed a novel antitubercular mechanism of of galangal\xa0through M. tuberculosis shikimate kinase (MtSK) inhibitory assays. Sequential extractions of nonpolar solvents hexane and dichloromethane (DCM) were performed on galangal and screened in MtSK inhibitory assays to identify potential activity. Samples were then subjected to high resolution (HR) LC-MS chemical fingerprinting and analysis. Additionally, a novel approach was undertaken for galangal using methods such as mass professional profiler (MPP) and global natural products social (GNPS) molecular networking for structure elucidation.