Ergosterol peroxide: an effect-directed detecting method from Anoectochilus elwesii and evaluation of anticancer activity.

Abstract

Ergosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecting method of HPTLC-DPPH/UHPLC/ESI-TOF-MS was developed for EP rapidly and precisely, providing an option for identifying oxidative compounds. EP exhibits high cytotoxic activity against SGC-7901 gastric cancer cells and the morphological apoptosis suggested that EP induced apoptosis and cell cycle arrest in G0/G1 phase. It could enhance the ROS level and cause a decrease in the mitochondrial membrane potential. Its antiproliferative mechanism is G0/G1 phase arrest and might be traced through ROS-mediated mitochondrial dysfunction pathways.