The effects of trypan blue use on the corneal endothelium during cataract surgery in patients with pseudoexfoliation syndrome (PEX)

Abstract

Abstract Purpose In the present clinical study, it was aimed to investigate the possible effects of Trypan blue (TB) use on the corneal endothelium during cataract surgery in eyes with pseudoexfoliation syndrome (PEX) during a three-month follow-up period using the contralateral eye control design. Methods This prospective, randomised controlled, individual cohort study included 92 eyes of 46 patients with bilateral PEX and cataracts. While 1% TB was applied to one eye of the patients before capsulorhexis (study group), it was not applied to the other eye (control group). Both groups were compared preoperatively and postoperatively in terms of endothelial cell density (ECD), endothelial cell loss (%), pleomorphism, polymegathism and central corneal thickness (CCT) using specular microscopy. Results Preoperative corneal ECD was measured as 2362.56\u2009±\u2009253.27 in the study group, 2380.84\u2009±\u2009220.54 in the control group, and 2145.58\u2009±\u2009221.71 in the study group and 2184.97\u2009±\u2009200.94 cells/mm2 in the control group in the postoperative 3rd-month follow-up (p\u2009=\u20090.71 and = 0.37, respectively). In addition, there were no significant differences between the two groups in terms of the percentage of hexagonal cells, coefficient of variation (CV), and CCT both preoperatively and postoperatively 3\u2009months later (p\u2009=\u20090.78, =0.39, =0.95 preoperatively and p\u2009=\u20090.31, =0.26, =0.83 postoperatively, respectively). Conclusion This study demonstrated that the injection of 1% TB into the anterior chamber for staining the anterior capsule during cataract surgery did not cause significant corneal endothelial changes at postoperative 3rd months, despite the increased fragility of corneal endothelial cells in patients with PEX.