Mitochondrial DNA Part B | 2019
The complete chloroplast genome of Biondia insignis Tsiang (Apocynaceae)
Abstract
Abstract The complete chloroplast genome from Biondia insignis Tsiang, a rare liana of the Apocynaceae endemic to China is determined in this study. The whole chloroplast genome sequence of Biondia insignis Tsiang has been characterized by Illumina pair-end sequencing. The circular genome is 160,381\u2009bp long, containing a large single copy region (LSC) of 91,493\u2009bp and a small single copy region (SSC) of 19,262\u2009bp, which are separated by a pair of 24,813\u2009bp inverted repeat regions (IRs). It encodes a total of 127 genes, including 73 protein-coding genes (70 PCG species), 46 tRNA genes (31 tRNA species), and eight ribosomal RNA genes (4 rRNA species). The most of gene species occur as a single copy, while 14 gene species occur in double copies. The overall A\u2009+\u2009T content is 62.2%, while the corresponding values of the LSC, SSC, and IR regions are 63.9, 68.0, and 56.7%, respectively. The evolutionary relationships revealed by phylogenetic analysis indicated that Biondia insignis Tsiang was relatively close to Vincetoxicum rossicum. These complete chloroplast genomes can be subsequently used for population genomic studies of Biondia and provide valuable insight into conservation and exploitation for this rare species.