Single-molecule sequencing yields the complete chloroplast genome sequence of Populus deltoides

Abstract

Abstract Populus deltoides is a fast-growing, large tree and one of the largest North American hardwood trees. In this study, the complete chloroplast (cp) genome sequence of P. deltoides is characterized. The whole cp genome was assembled to 156,867\u2009bp, including a large single copy (LSC) region of 85,534\u2009bp, a small single copy (SSC) region of 16,513\u2009bp and a pair of inverted repeats (IRs) region of 27,410\u2009bp. The base content of the P. deltoides cp genome is A (32.0%), T (31.3%), C (18.0%), and G (18.7%), and AT bases occupy a large proportion of the cp genome. The neighbor-joining phylogenetic analysis with 20\u2009cp genomes from the Salicaceae family showed that P. deltoides is sister to Populus davidiana. These will provide for the evolutionary and biological studies in Salicaceae family.