The Specialist Marine Herbivore Elysia papillosa Grows Faster on a Less Utilized Algal Diet

Abstract

Many small specialist herbivores utilize their food resources both for nutrition and as a structural refuge or resource. Trophic linkage cannot solely be inferred from physical association of herbivores with a potential food item, because herbivores may temporarily inhabit algae or plants on which they do not feed. Elysia papillosa, a small sacoglossan sea slug, consumes and sequesters chloroplasts from the siphonaceous, chlorophytic alga Penicillus capitatus; it also maintains moderate densities on this alga. Recently, E. papillosa was also infrequently found in association with the alga Penicillus lamourouxii, which displays density similar to that of P. capitatus. After collecting E. papillosa from each of the two algal species from a shallow-water site along the west central coast of Florida, we used DNA barcoding of the rbcL gene sequences in order to determine whether the slug was consuming both algal species. The molecular data indicated that E. papillosa consumed and sequestered chloroplasts from the same algal species from which they were collected. A laboratory feeding experiment tested whether algal diet (P. capitatus or P. lamourouxii) had an impact on slug growth rate as measured by change in body size (mm). After 3 weeks E. papillosa fed P. lamourouxii achieved a mean body length that was 1.5–2 times that recorded for slugs fed P. capitatus, but maximum growth depended on the original field host. Thus, while the highest densities of E. papillosa in the field occurred on P. capitatus, slugs grew much faster on P. lamourouxii in the laboratory. The observed association of E. papillosa with P. capitatus must be related to other factors, such as foraging efficiency, algal morphology, algal biochemistry, or algal suitability as a refuge.