Isolation, screening, and molecular identification of lignin-degrading fungi isolates from Cibinong Science Centre, Indonesia Institute of Sciences, Cibinong, West Java

Abstract

Lignin is a recalcitrant compound that has potential as fuels and chemicals on industries. Contamination of recalcitrant compound with the lignin-like structure on the ecosystem is being concerned worldwide. Fungi is the most targeted organisms with lignin-degrading ability, can secrets laccase and peroxidase to facilitate free radical chain reaction on lignin complex structure. Isolation, screening and identification for isolate with ligninolytic ability is the main purpose of this project. Phylum Ascomycota and Basidiomycota be the main target of this research. Isolation technics that being used are surface sterilization with distilled water and NaClO on PDA medium. Pure culture with ability to grow on lignin minimal medium with black liquor as the only carbon sources then are identified by molecular identification. Isolates that can grow on lignin minimal medium are identified as Fusarium verticillioides strain CBS 127178, Lasiodiplodia sp. LAS-2016 strain CBS 125262, Trichoderma harzianum strain 35814DRJ, and Lasiodiplodia theobromae strain CBS 127106. Four isolates obtained with lignin-degrading ability then preserved by cryopreservation technic for use in the next projects.