Comparative Analysis Of In Vitro Antidiabetic Activity Of Wild And Micropropagated Solanum Trilobatum L

Abstract

Diabetes is metabolic disorder which is characterized by an increase in glucose level due to a relative deficiency of insulin. Recent decades have experienced a very high prevalence of diabetes mellitus. One way of treating diabetes is to reduce gastrointestinal glucose production by inhibiting carbohydrate digesting enzymes called pancreatic alpha-amylase and intestinal alpha-glucosidase. The present study aims to compare the efficacy of wild and micropropagated Solanum trilobatum for its in vitro antidiabetic property. Solanum trilobatum grown in vitro under aseptic conditions was compared with wild-grown, and the results have revealed that both wild and micropropagated plants have effective dose-dependent inhibition of alpha-amylase and alpha-glucosidase enzyme. For both alpha-amylase and alpha-glucosidase assay micropropagated plant has shown lesser efficacy at all concentrations when compared to that of wild grown. still micropropagated showed effective inhibition with nearly 90% similarity.