Biodiesel production from Pichia Kudriavzevii using Algae Kappaphycus Alvarezii as a fermentation substrate

Abstract

Kappaphycus alvarezii is red algae that can be used as an alternative raw material in biodiesel production, because K. alvarezii c ontains high carbohydrate which is 60%. This study aims to determine the effect of nutrition and fermentation duration of K. alvarezii algae to hydrolysis results on cell s biomass. This study used the enzymatic hydrolysis method, fermentation method and sonication method. In this study, hydrolysis method was conducted by using 150 KNU/L of α-amylase enzyme with 0.5%, 1%, 1.5% and 2% of algae concentration. K. alvarezii algae is fermented by yeast Phicia kudriavzevii. This study, fermentation method used was Gandasil-D® as an inorganic nutrition and Yeast Extract as an organic nutrition. The result of this research shows that P. kudriavzevii biomass can produced biodiesel by carbon source from K. alvarezii algae on 48th hours. The dominant types of fatty acids are oleic acid which is 39.33%.The result shows that it has high potential to be used as biodiesel raw material.