Reproductive Performance of Java Barb (Punctius javanicus) Injected sGNRH and Domperidone of Different Dosage

Abstract

Java barb (P. javanicus) is freshwater fish frequently found in Java and Sumatra waters of Indonesia. Java Barb has a seasonal spawning cycle. Accordingly, the production of java barb seeds is limited by season. Therefore, it is necessary to research on hormonal manipulating systems of reproduction to needs of seeds. This study aimed to determine the effect of sGnRH and domperindone on Java barb reproductive performance. There were 12 female and 12 male java barb broodstock, with 400-500 grams and 300-400 grams bodyweight respectively. The broodstock used on third stage of gonadal maturation. This study used an experimental method with a completely randomized design (CRD) consisting of 4 treatments and 3 replicates. Mixture of GnRH and domperidone was injected intramuscularly. The dosage treatments used were dosage A (control), treatment B (0.3 ml/kg), treatment C (0.4 ml/kg) and treatment D (0.5 ml/kg). The data gathered included latency period, fertilization rate (FR), hatching rate (HR), survival rate (SR) and water quality. The results showed that the injection of sGnRH and domperidone produced different latency periods, significantly affected (P <0.05) fertilization rate (FR) and hatching rate (HR), but had no significant effect on survival (SR). The best results were obtained at treatment. B and treatment C with 0.3 ml/kg and 0,4 ml/kg dosage resulting in 9-12 hours latency period, 80,50±5,57 to 84.33 ± 5.51% of Fertilization Rate (FR), 68,83±6,81 to 71.17 ± 5.35% Hatching Rate (HR) and 53,85±2,35 to 60.69 ± 10.66% of Survival Rate (SR).