Detection of Tetracycline tet(k) Gene in Clinical Staphylococcus aureus Isolates

Abstract

This paper concerned with the identification of S .aureus and detection of tet (k) gene using PCR technique .A total of fifty samples were collected from different clinical sources: 20 from burns, (10) surgical wounds, (10) dental carries, and (10) urine samples from patients who were admitted to West Erbil Emergency and Rizgary teaching Hospitals during 1 October to 17december 2020. Isolates were identified using conventional method and confirmed by VITEK2 compact system. Twenty out of Fifty isolates were identified as Staphylococcus aureus. Disk diffusion method was done for antibiotic resistance, all S. aureus isolates were showed resistance to antibiotic in different percentage: 100% for AMC, AP, AX, and PG, 90% for ME, 65% for CAZ, 65% for TM, 60% for T, 60% for E, 50% for CRO, 45% for CTX, and S, 45% for L, 40% for RA, 40% for DA, 35% for CIP, 35% for C%15, for KF and 10% for GM while all isolates were sensitive to vancomycin . PCR technique results of partial fragments of tet (K) gene using 360bp primer were shown that 7 (35%) of isolates were tetk positive, while 13 (65%) of the isolates were tetk negative.