The role of microbes in organic material decomposition and formation of compost bacterial communities

Abstract

The application of microbial decomposers and bio-stimulant into agriculture wastes influence the abundance of microorganisms and their biological diversity of the compost. The study was conducted to determine the role of microbial in the decomposition of agricultural wastes that produces compost and its effect on the diversity and function of microbes that inhabited the compost product. Agricultural wastes used straw, leaves and grass from the Cibinong Science Center-Botanical Garden. Decomposer microbes used fungi (Trichoderma), and bacteria (Azospirillum brasilense and Agrobacterium sp.). The composting process was conducted anaerobically and the compost beds were reversed every 7 days. The diversity and function of inhabiting microbes resulted from the composting process was determined and identified by conducting microbial isolation and 16S rRNA genes identification. The ability of the microbes as decomposers was also analyzed. The results showed that the genus Bacillus tended to dominate. The microbial consortia have a high enzyme activity to degrade material waste agriculture. Of the three sources of compost used, the grass produced a better compost, forming a diverse and beneficial bacterial community. Therefore, it can be stated that the use of microbial decomposers for composting the agriculture wastes is an appropriate method for forming the beneficial microbial ecosystems.