The activity of Flavonoid Isolates from Papaya (Carica papaya L.) Seed as Pancreatic Lipase Inhibitor

Abstract

Excessive fat consumption can cause obesity, which is currently one of the global problems, because it can trigger various diseases such as coronary heart disease, diabetes, and so on. Absorption of fat into the body requires pancreatic lipase enzymes, which can be reduced by lipase inhibitors such as orlistat drugs. Previous research has shown that the extract of papaya seed, which contains many flavonoids, was able to inhibit pancreatic lipase. Therefore, the aims of this study are to isolate and identify the flavonoid compounds from papaya seeds that are active as pancreatic lipase inhibitors. Papaya seed powder was extracted using ethanol 70%, followed by isolation using TLC, identification by phytochemical test and UV and IR spectroscopy, while pancreatic lipase inhibitory activity has been done using titrimetric method. To confirm the Inhibitory activity of the predicted flavonoid against the enzyme, also had been done in silico study. The results had shown that there are two types of flavonoid in the ethanol extract of papaya seed, that are active as pancreatic lipase inhibitors. The two flavonoid isolates have inhibition power of 132 and 12 times stronger than orlistat in the same mass. Result of UV-VIS and IR spectrum has shown that both isolates were suspected to have a similar structure to the three flavonoid compounds: epicatechin, catechin or epigallocatechin-3-gallate (EGCG). In silico study, also confirmed that each of the predicted flavonoids has higher binding affinity against pancreatic lipase, than orlistat.